<div dir="ltr">Wow! Thank you for doing this Louis!<div><br></div><div>Craig</div></div><div class="gmail_extra"><br><br><div class="gmail_quote">On Mon, Jan 27, 2014 at 4:25 PM, Louis Huang <span dir="ltr"><<a href="mailto:louis.huang@aegia.nu" target="_blank">louis.huang@aegia.nu</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hey all,<br>
<br>
Here is the video on DIY Molecular Design & Cloning by Craig that was held by CounterCultureLabs at sudoroom on 1/14/14. Video can be found here:<br>
<a href="http://www.youtube.com/watch?v=kKiqh-FhyNo" target="_blank">http://www.youtube.com/watch?<u></u>v=kKiqh-FhyNo</a><br>
<br>
<br>
Lecture details a basic overview of designing and cloning microorganisms using open access databases.<br>
        Subjects discussed include:<br>
<br>
                - Primer design -- tricks, tools and resources<br>
                - PCR-based cloning -- methods and means for the DIYer<br>
                - Purification, Isolation and Verification<br>
                - Databases and software used for design and cloning<br>
<br>
<br>
Join us for part II of this lecture series on cloning. Craig Rouskey will talk in depth on PCR:<br>
<br>
        a. PCR Principle<br>
        b. Components/role of each component<br>
        c. setting up PCR/Making adjustments to reagents<br>
        d. making PCR buffers (Maniatis)<br>
        e. Reconstituting Primers<br>
        f. Working the thermocycler<br>
<br>
Sign up for the event here: <a href="http://www.meetup.com/Counter-Culture-Labs/events/161499622/" target="_blank">http://www.meetup.com/Counter-<u></u>Culture-Labs/events/161499622/</a><br>
Held on 2/11/14 6:30PM @ sudoroom<br>
<br>
For more CounterCultureLabs events visit the meet up schedule @ <a href="http://www.meetup.com/Counter-Culture-Labs/" target="_blank">http://www.meetup.com/Counter-<u></u>Culture-Labs/</a><br>
<br>
Happy sciencing,<br>
Louis<span class="HOEnZb"><font color="#888888"><br>
<br>
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