Hey all,
Here is the video on DIY Molecular Design & Cloning by Craig that was held by CounterCultureLabs at sudoroom on 1/14/14. Video can be found here:
http://www.youtube.com/watch?v=kKiqh-FhyNo
Lecture details a basic overview of designing and cloning microorganisms using open access databases.
Subjects discussed include:
- Primer design -- tricks, tools and resources
- PCR-based cloning -- methods and means for the DIYer
- Purification, Isolation and Verification
- Databases and software used for design and cloning
Join us for part II of this lecture series on cloning. Craig Rouskey will talk in depth on PCR:
a. PCR Principle
b. Components/role of each component
c. setting up PCR/Making adjustments to reagents
d. making PCR buffers (Maniatis)
e. Reconstituting Primers
f. Working the thermocycler
Sign up for the event here: http://www.meetup.com/Counter-Culture-Labs/events/161499622/
Held on 2/11/14 6:30PM @ sudoroom
For more CounterCultureLabs events visit the meet up schedule @ http://www.meetup.com/Counter-Culture-Labs/
Happy sciencing,
Louis
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