Hi Keoni - do you have any pipettes for the OT-2? 

We installed it right on the boundary between CCL and Sudo for now. Once we start doing real experiments, we should move it into the wetlab though. 

We also happen to have a Biometra TRobot, which is essentially a thermocycler with an automated lid, so it can be easily incorporated into a liquid handling robot. That should give us an ideal platform for PCR applications on the Opentrons...

DNA barcoding of mushroom species is probably our #1 killer app for this robot. When Alan Rockefeller comes back from his current outing throughout Mexico, he will likely bring several hundred mushroom samples with him, all of which will need to be submitted to PCR amplification and Sanger sequencing. Alan usually mashes each sample with a little plastic pestle in an Eppendorf tube - not sure how to replicate that on the robot, but we could brainstorm a little device to macerate mushroom samples...

Patrik

On Sun, Sep 30, 2018 at 6:42 PM Keoni Gandall <koeng101@gmail.com> wrote:
The hardware issue should be easily solved with a little hack that deactivates OpenTrons pipette-verification (it worked before a certain software update). 

The hope is that the robot can help bridge the gap between the software folks at SudoRoom and wetware folks in CCL.

I'll at hands on workshop as well - come find me in the red polo if you'd like to talk about the machine!

On Sun, Sep 30, 2018 at 6:34 PM Patrik D'haeseleer <patrikd@gmail.com> wrote:
Hi all,

You may have noticed that Keoni Gandall dropped of a brand new (ish) OpenTrons OT-2 liquid handling robot recently! This is the much smaller and newer sibling to the giant 5'x5' robot CCL has in the basement. It is currently sitting in SudoRoom, and may or may not have a hardware or electrical issue to be diagnosed first.

Also, if you'd like to learn how to use this machine, OpenTrons is running a FREE HANDS-ON WORKSHOP to learn how to use the OT-2 tomorrow at the Mission Bay Conference Center; Monday 2-4pm. Registration is free, but space may run out, so I would recommend you sign up asap:


Patrik