Difference between revisions of "Phage therapy"

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:a. Swab patients, grow samples on Modified Thayer Martin medium, broth cultures grown in Trypticase Soy Broth.
:a. Swab patients, grow samples on Modified Thayer Martin medium, broth cultures grown in Trypticase Soy Broth.
:b. Supernatants harvested and filtered through a 0.2um filter and analyzed by SDS-PAGE.
:b. Supernatants harvested and filtered through a 0.2um filter and analyzed by SDS-PAGE.
:c.  
:c. Characterize phage via PCR
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5.Develop methods by which bacteriophages can be cultured and used as prophylaxis and therapy for gonoccocal infections.
5.Develop methods by which bacteriophages can be cultured and used as prophylaxis and therapy for gonoccocal infections.
:a. Test and optimize growth conditions for strains of antibiotic resistant gonorrhea from which bacteriophage can be isolated.
:b. Harvest phage and perform proof-of-concept, in vitro experiments in which gonorrhea is lysed with preparations of bacteriophage.


== Experiments and Anticipated Problems ==
== Experiments and Anticipated Problems ==
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