Difference between revisions of "Phage therapy"

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:'''Craig Rouskey''', MSc in Molecular Biology, Biochemistry, Microbiology and Immunology
:'''Craig Rouskey''', MSc in Molecular Biology, Biochemistry, Microbiology and Immunology
:'''Ami Knop Ullrich''', MSc in Medical Microbiology and Immunology
:'''Ami Knop Ullrich''', MSc in Medical Microbiology and Immunology
:'''Nina DiPrimio''', PhD in Pharmacology
 
:'''Louis Huang''', BSc in Chemical Engineering and Economics
:'''Marc Juul''', MSc in Biotechnology Engineering
:'''Jenny Ryan''', MA in Anthropology, MA in Communication
:'''Tess Westbrook''', BSc Biology, MBA
:'''Ianto Lin Xi''', BSc Molecular Biology


== Introduction ==
== Introduction ==
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== Questions and Specific Aims ==
== Questions and Specific Aims ==
[[Experiments]]
:'''Pilot Project Aims'''
:'''Pilot Project Aims'''
<br />
<br />
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#Addressing previous citizen science failures and conducting research with a greater adherence to quality.
#Addressing previous citizen science failures and conducting research with a greater adherence to quality.
#Preventing Lysogeny.
#Preventing Lysogeny.
== Experiments ==
:Samples will be grown on Modified Thayer Martin agar supplemented with antibiotics. Colonies will '''(1)'''be stored in glycerol at -80oC, '''(2)'''grown in liquid culture for the assessment of bacteriophage production, '''(3)'''grown in Maltose to ensure we have cultured ''N. gonorrhoeae,'' and '''(4)'''grown in Fastidious Broth with 3rd-generation antibiotics to assess antibiotic resistance.
:
:Following growth in FB, supernatants will be analyzed for the presence of bacteriophage using SDS-PAGE electrophoresis.
:
:If bacteriophage proteins are present in the supernatants, these cultures will be re-grown in bulk and bacteriophage-containing sups will be stored at -80oC.
:
:All clinical screening data will be available to patients online through our website. If patients are positive, they will be asked to fill out a form on their results page that will detail their risk level. This data is important in the surveillance of antibiotic-resistant ''N. gonorrhoeae'' emergence. '''''All screening procedures and results are anonymous.'''''


===Protocol Documentation===
===Protocol Documentation===
http://aegia.nu/130721_prephage-protocols.txt
http://aegia.nu/folder/getitproject/130721_prephage-protocols.txt


== Laboratory Needs ==
== Laboratory Needs ==
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== References ==
== References ==
<references />
<references />
[[Category: Health]]
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