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| :'''Craig Rouskey''', MSc in Molecular Biology, Biochemistry, Microbiology and Immunology | | :'''Craig Rouskey''', MSc in Molecular Biology, Biochemistry, Microbiology and Immunology |
| :'''Ami Knop Ullrich''', MSc in Medical Microbiology and Immunology | | :'''Ami Knop Ullrich''', MSc in Medical Microbiology and Immunology |
| :'''Nina DiPrimio''', PhD in Pharmacology
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| :'''Louis Huang''', BSc in Chemical Engineering and Economics
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| :'''Marc Juul''', MSc in Biotechnology Engineering
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| :'''Jenny Ryan''', MA in Anthropology, MA in Communication
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| :'''Tess Westbrook''', BSc Biology, MBA
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| :'''Ianto Lin Xi''', BSc Molecular Biology
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| == Introduction == | | == Introduction == |
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| == Questions and Specific Aims == | | == Questions and Specific Aims == |
| | [[Experiments]] |
| :'''Pilot Project Aims''' | | :'''Pilot Project Aims''' |
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| #Addressing previous citizen science failures and conducting research with a greater adherence to quality. | | #Addressing previous citizen science failures and conducting research with a greater adherence to quality. |
| #Preventing Lysogeny. | | #Preventing Lysogeny. |
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| == Experiments ==
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| :Samples will be grown on Modified Thayer Martin agar supplemented with antibiotics. Colonies will '''(1)'''be stored in glycerol at -80oC, '''(2)'''grown in liquid culture for the assessment of bacteriophage production, '''(3)'''grown in Maltose to ensure we have cultured ''N. gonorrhoeae,'' and '''(4)'''grown in Fastidious Broth with 3rd-generation antibiotics to assess antibiotic resistance.
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| :Following growth in FB, supernatants will be analyzed for the presence of bacteriophage using SDS-PAGE electrophoresis.
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| :If bacteriophage proteins are present in the supernatants, these cultures will be re-grown in bulk and bacteriophage-containing sups will be stored at -80oC.
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| :All clinical screening data will be available to patients online through our website. If patients are positive, they will be asked to fill out a form on their results page that will detail their risk level. This data is important in the surveillance of antibiotic-resistant ''N. gonorrhoeae'' emergence. '''''All screening procedures and results are anonymous.'''''
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| ===Protocol Documentation=== | | ===Protocol Documentation=== |
| http://aegia.nu/130721_prephage-protocols.txt | | http://aegia.nu/folder/getitproject/130721_prephage-protocols.txt |
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| == Laboratory Needs == | | == Laboratory Needs == |
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| == References == | | == References == |
| <references /> | | <references /> |
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| | [[Category: Health]] |