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Difference between revisions of "Phage therapy"
→Questions and Specific Aims
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:a. Swab patients, grow samples on Modified Thayer Martin medium, broth cultures grown in Trypticase Soy Broth. | :a. Swab patients, grow samples on Modified Thayer Martin medium, broth cultures grown in Trypticase Soy Broth. | ||
:b. Supernatants harvested and filtered through a 0.2um filter and analyzed by SDS-PAGE. | :b. Supernatants harvested and filtered through a 0.2um filter and analyzed by SDS-PAGE. | ||
:c. | :c. Characterize phage via PCR | ||
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5.Develop methods by which bacteriophages can be cultured and used as prophylaxis and therapy for gonoccocal infections. | 5.Develop methods by which bacteriophages can be cultured and used as prophylaxis and therapy for gonoccocal infections. | ||
:a. Test and optimize growth conditions for strains of antibiotic resistant gonorrhea from which bacteriophage can be isolated. | |||
:b. Harvest phage and perform proof-of-concept, in vitro experiments in which gonorrhea is lysed with preparations of bacteriophage. | |||
== Experiments and Anticipated Problems == | == Experiments and Anticipated Problems == | ||