[Biohackers] [DIYbio] Re: Open source centrifuge!

Nathan McCorkle nmz787 at gmail.com
Fri Sep 27 11:23:54 PDT 2013


Harbor Freight (mostly chinese-imported tool store across USA) sells
dremel-like tools for $9 with accessories, not to mention the always
available %20 off coupons on retailmenot.com

On Thu, Sep 26, 2013 at 11:14 AM, Matthew Harbowy <hbergeronx at gmail.com> wrote:
> In most cases, a 2000g, 6000 rpm minifuge is all you need for mini preps and
> pelleting DNA. Galaxy VWR minifuge for this is $422, brand new, from VWR.
> This kit is $260. You can pick up minifuges at used resellers for <$50. A
> basic Dremel tool is about $50. A replacement rotor for the galaxy microfuge
> (hint, hint) is $70.
>
> To Patrik's point: how much is your eyesight worth? Be careful out there.
> When making plastic covers, use highly resistant materials, like
> polycarbonate laminates (e.g. MAKROLON). Metal rotors are probably a better
> idea than plastic ones. IMO, the brand new minifuge is not so expensive and
> has clearly traceable product liability. DIY in this case might be a great
> learning tool, if by "losing an eye" you learn what not to do.
>
> This has been your "Important safety tip there, Egan" of the day.
>
> Matt
>
> On Thursday, September 26, 2013, Patrik D'haeseleer wrote:
>>
>> I would also want to see what happens to that thin acrylic cover when a
>> fully laden tube ejects from the rotor at full speed. High speed centrifuges
>> are explosion-prone devices, and need to be tested as such. Flying shards of
>> acrylic are no fun...
>>
>> The typical recommendation for using the dremelfuge are to hold it inside
>> a metal cooking pot - at least that will give you some serious protection.
>>
>> Patrik
>>
>>
>> On Thu, Sep 26, 2013 at 10:17 AM, Craig Rouskey <craigrouskey at gmail.com>
>> wrote:
>>>
>>> Thanks Jenny! Typically for DNA work centrifuge speeds need to be approx.
>>> 13000rpm/18000xg
>>>>>> Craig J. Rouskey
>>> contact at craigrouskey.com
>>> http://www.craigrouskey.com
>>>
>>>
>>> On Thu, Sep 26, 2013 at 1:15 AM, Jenny Ryan <tunabananas at gmail.com>
>>> wrote:
>>>>
>>>> Via @BiologiGaragen: New open source centrifuge
>>>> http://t.co/otDqV44UER http://t.co/54l7f5DNOt
>>>> Shared via TweetCaster
>>>
>>>
>>>
>>> _______________________________________________
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>>>
>>
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-- 
-Nathan


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