[Biohackers] Open source centrifuge!

[Craig Rouskey]

True, you can precipitate DNA with a slower speed, you would just have to spin for much longer making high throughput preps less efficient. For a project like GETit we'd want to have a more efficient fuge.

—
Craig J. Rouskey
contact at craigrouskey.com
http://www.craigrouskey.com

On Thu, Sep 26, 2013 at 11:14 AM, Matthew Harbowy <hbergeronx at gmail.com>
wrote:

> In most cases, a 2000g, 6000 rpm minifuge is all you need for mini preps
> and pelleting DNA. Galaxy VWR minifuge for this is $422, brand new, from
> VWR. This kit is $260. You can pick up minifuges at used resellers for
> <$50. A basic Dremel tool is about $50. A replacement rotor for the galaxy
> microfuge (hint, hint) is $70.
> To Patrik's point: how much is your eyesight worth? Be careful out there.
> When making plastic covers, use highly resistant materials, like
> polycarbonate laminates (e.g. MAKROLON). Metal rotors are probably a better
> idea than plastic ones. IMO, the brand new minifuge is not so expensive and
> has clearly traceable product liability. DIY in this case might be a great
> learning tool, if by "losing an eye" you learn what not to do.
> This has been your "Important safety tip there, Egan" of the day.
> Matt
> On Thursday, September 26, 2013, Patrik D'haeseleer wrote:
>> I would also want to see what happens to that thin acrylic cover when a
>> fully laden tube ejects from the rotor at full speed. High speed
>> centrifuges are explosion-prone devices, and need to be tested as such.
>> Flying shards of acrylic are no fun...
>>
>> The typical recommendation for using the dremelfuge are to hold it inside
>> a metal cooking pot - at least that will give you some serious protection.
>>
>> Patrik
>>
>>
>> On Thu, Sep 26, 2013 at 10:17 AM, Craig Rouskey <craigrouskey at gmail.com<javascript:_e({}, 'cvml', 'craigrouskey at gmail.com');>
>> > wrote:
>>
>>> Thanks Jenny! Typically for DNA work centrifuge speeds need to be approx.
>>> 13000rpm/18000xg
>>> —
>>> Craig J. Rouskey
>>> contact at craigrouskey.com <javascript:_e({}, 'cvml',
>>> 'contact at craigrouskey.com');>
>>> http://www.craigrouskey.com
>>>
>>>
>>> On Thu, Sep 26, 2013 at 1:15 AM, Jenny Ryan <tunabananas at gmail.com<javascript:_e({}, 'cvml', 'tunabananas at gmail.com');>
>>> > wrote:
>>>
>>>> Via @BiologiGaragen: New open source centrifuge
>>>> http://t.co/otDqV44UER http://t.co/54l7f5DNOt
>>>> Shared via TweetCaster
>>>>
>>>
>>>
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>>
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