[sudo-discuss] New OpenTrons OT-2 in Sudo/CCL!

[Keoni Gandall]

Hey Patrik,

I have a single p10 and a broken multichannel 300ul that I'll bring over (I
bet you may be able to fix the multichannel 300ul).

I'll also think of a good way to automate maceration of mushrooms. I feel
like it may be a bit difficult due to cross-contamination issues.

Keoni

On Thu, Oct 4, 2018 at 3:58 AM Patrik D'haeseleer <patrikd at gmail.com> wrote:

> Hi Keoni - do you have any pipettes for the OT-2?
>
> We installed it right on the boundary between CCL and Sudo for now. Once
> we start doing real experiments, we should move it into the wetlab though.
>
> We also happen to have a Biometra TRobot, which is essentially a
> thermocycler with an automated lid, so it can be easily incorporated into a
> liquid handling robot. That should give us an ideal platform for PCR
> applications on the Opentrons...
>
> DNA barcoding of mushroom species is probably our #1 killer app for this
> robot. When Alan Rockefeller comes back from his current outing throughout
> Mexico, he will likely bring several hundred mushroom samples with him, all
> of which will need to be submitted to PCR amplification and Sanger
> sequencing. Alan usually mashes each sample with a little plastic pestle in
> an Eppendorf tube - not sure how to replicate that on the robot, but we
> could brainstorm a little device to macerate mushroom samples...
>
> Patrik
>
> On Sun, Sep 30, 2018 at 6:42 PM Keoni Gandall <koeng101 at gmail.com> wrote:
>
>> The hardware issue should be easily solved with a little hack that
>> deactivates OpenTrons pipette-verification (it worked before a certain
>> software update).
>>
>> The hope is that the robot can help bridge the gap between the software
>> folks at SudoRoom and wetware folks in CCL.
>>
>> I'll at hands on workshop as well - come find me in the red polo if you'd
>> like to talk about the machine!
>>
>> On Sun, Sep 30, 2018 at 6:34 PM Patrik D'haeseleer <patrikd at gmail.com>
>> wrote:
>>
>>> Hi all,
>>>
>>> You may have noticed that Keoni Gandall dropped of a brand new (ish) OpenTrons
>>> OT-2 liquid handling robot <http://opentrons.com/ot-2>recently! This is
>>> the much smaller and newer sibling to the giant 5'x5' robot CCL has in the
>>> basement. It is currently sitting in SudoRoom, and may or may not have a
>>> hardware or electrical issue to be diagnosed first.
>>>
>>> Also, if you'd like to learn how to use this machine, OpenTrons is
>>> running a *FREE HANDS-ON WORKSHOP* to learn how to use the OT-2
>>> tomorrow at the Mission Bay Conference Center; Monday 2-4pm. Registration
>>> is free, but space may run out, so I would recommend you sign up asap:
>>>
>>>
>>> https://www.eventbrite.com/e/opentrons-hands-on-workshop-tickets-49398612580
>>>
>>> Patrik
>>>
>>
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