Difference between revisions of "Phage therapy"

Phage therapy (view source)

Revision as of 07:04, 25 July 2013

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→‎Questions and Specific Aims

CRouskey

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-:'''Specific Aims'''
+:'''Project Aims'''
 <br />
-. Begin a long-term mobile screening program for antibiotic resistant gonorrhea on the streets of Oakland and San Francisco.<br />
+. Monitor the emergence of antibiotic resistant gonorrhea.<br />
-:a. Develop a website through which patients, after being provided a patient number can retrieve their results with a full analysis of their strain.
+:a. Compose and deploy a mobile screening unit at various locations throughout Oakland and San Francisco.
-:b. Compose and deploy a mobile screening unit at various locations throughout Oakland and San Francisco.
+:b. Compile data from the mobile screening program and integrate it with data from other public health organizations.
 <br />
-. Support the Center for Disease Control's efforts to monitor the emergence of antibiotic resistant gonorrhea.
+. Develop a community laboratory space in Oakland, CA that has the equipment we need in which we can conduct our research.
-:a. Compile data from the mobile screening program and report it (anonymously) to the CDC.
-:b. Send antibiotic-resistant gonorrhea samples to the center for disease control according to the Instructions for Submitting Specimens to CDC Gonorrhea Laboratory for Confirmation Testing and/or Testing of Clinical Treatment Failures.
-<br />
-. Develop a community laboratory space in Oakland, CA that has the equipment (acquired or built) we need in which we can conduct our research.
 :a. Procure capital equipment and reagents needed for this project.
 :b. Procure reagents and equipment to begin molecular microbiological research.
-:c. Open space up to provide education for Oakland teens.
 <br />
 . Isolate, identify and characterize the first known bacteriophages from '''N. gonorrhoeae.'''
-:a. Swab patients, grow samples on Modified Thayer Martin medium, broth cultures grown in Trypticase Soy Broth.
+:a. Harvest samples from our mobile screening program.
-:b. Supernatants harvested and filtered through a 0.2um filter and analyzed by SDS-PAGE.
+:b. Perform Genomic and Proteomic Analyses on Bacteriophage samples.
-:c. Characterize phage via PCR
 <br />
 .Develop methods by which bacteriophages can be cultured and used as prophylaxis and therapy for gonoccocal infections.
 :a. Test and optimize growth conditions for strains of antibiotic resistant gonorrhea from which bacteriophage can be isolated.
 :b. Harvest phage and perform proof-of-concept, in vitro experiments in which gonorrhea is lysed with preparations of bacteriophage.
+<br />
+. Provide educational outreach opportunties.
+:a. Provide information on prevention and treatment of gonorrhea.
+:b. Provide in-the-lab training and experience for those with the desire to pursue citizen science.
 == Anticipated Problems and Solutions ==