Difference between revisions of "Phage therapy"
→Questions and Specific Aims
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:'''Specific Aims''' | :'''Specific Aims''' | ||
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1. Begin a long-term mobile screening program for antibiotic resistant gonorrhea on the streets of Oakland and San Francisco.<br /> | |||
:a. Develop a website through which patients, after being provided a patient number can retrieve their results with a full analysis of their strain. | |||
:b. Compose and deploy a mobile screening unit at various locations throughout Oakland and San Francisco. | |||
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2. Support the Center for Disease Control's efforts to monitor the emergence of antibiotic resistant gonorrhea. | |||
:a. Compile data from the mobile screening program and report it (anonymously) to the CDC. | |||
:b. Send antibiotic-resistant gonorrhea samples to the center for disease control according to the Instructions for Submitting Specimens to CDC Gonorrhea Laboratory for Confirmation Testing and/or Testing of Clinical Treatment Failures. | |||
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3. Develop a community laboratory space in Oakland, CA that has the equipment (acquired or built) we need in which we can conduct our research. | |||
:a. Procure capital equipment and reagents needed for this project. | |||
:b. Procure reagents and equipment to begin molecular microbiological research. | |||
:c. Open space up to provide education for Oakland teens. | |||
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4. Isolate, identify and characterize the first known bacteriophages from '''N. gonorrhoeae.''' | |||
:a. Swab patients, grow samples on Modified Thayer Martin medium, broth cultures grown in Trypticase Soy Broth. | |||
:b. Supernatants harvested and filtered through a 0.2um filter and analyzed by SDS-PAGE. | |||
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5.Develop methods by which bacteriophages can be cultured and used as prophylaxis and therapy for gonoccocal infections. | |||
== Experiments and Anticipated Problems == | == Experiments and Anticipated Problems == | ||