Difference between revisions of "Phage therapy"

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:'''Specific Aims'''
:'''Specific Aims'''
# Provide surveillance data on the emergence of antibiotic-resistant ''N. gonorrhoeae.''
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# Uncover lytic bacteriophages from clinical strains of ''N. gonorrhoeae.''
1. Begin a long-term mobile screening program for antibiotic resistant gonorrhea on the streets of Oakland and San Francisco.<br />
# Characterize lytic pathways of novel ''N. gonorrhoeae'' bacteriophage.
:a. Develop a website through which patients, after being provided a patient number can retrieve their results with a full analysis of their strain.
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:b. Compose and deploy a mobile screening unit at various locations throughout Oakland and San Francisco.
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2. Support the Center for Disease Control's efforts to monitor the emergence of antibiotic resistant gonorrhea.
:a. Compile data from the mobile screening program and report it (anonymously) to the CDC.
:b. Send antibiotic-resistant gonorrhea samples to the center for disease control according to the Instructions for Submitting Specimens to CDC Gonorrhea Laboratory for Confirmation Testing and/or Testing of Clinical Treatment Failures. 
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3. Develop a community laboratory space in Oakland, CA that has the equipment (acquired or built) we need in which we can conduct our research.
:a. Procure capital equipment and reagents needed for this project.   
:b. Procure reagents and equipment to begin molecular microbiological research.
:c. Open space up to provide education for Oakland teens.
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4. Isolate, identify and characterize the first known bacteriophages from '''N. gonorrhoeae.'''
:a. Swab patients, grow samples on Modified Thayer Martin medium, broth cultures grown in Trypticase Soy Broth.
:b. Supernatants harvested and filtered through a 0.2um filter and analyzed by SDS-PAGE.
:c.  
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5.Develop methods by which bacteriophages can be cultured and used as prophylaxis and therapy for gonoccocal infections.


== Experiments and Anticipated Problems ==
== Experiments and Anticipated Problems ==
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