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Difference between revisions of "Open Biotech/PCR"
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= Materials = | |||
== Heating == | |||
*[http://en.wikipedia.org/wiki/Nichrome Nichrome wire] | |||
* [http:// | |||
* | == PCR tube holder == | ||
* | |||
=== PCR tube dimensions === | |||
* [http:// | *Height: 20.81 (± 0.07) to 20.93 (± 0.08) mm | ||
*Diameter: 5.95 (± 0.04) to 6.07 (± 0.03) mm | |||
:Source: [http://www.spartanbio.com/wp-content/themes/spartan/assets/application_notes/17.pdf Dimensions and thermal property of different PCR tubes] | |||
Note that many PCR machines have PCR tube holes that are much shallower than the height of a PCR tube. | |||
The curvature/slant of the bottom runs to about half-way up the tube. | |||
=== Possible materials === | |||
Aluminum has a high thermal conductivity and is easy to machine. Adding a chunk of metal will slow temperature change. This is not desirable for rapid cycling, but it will make it easier to ensure that the temperature at the temperature probe and the temperature in the PCR tubes is the same. | |||
Using a holed sheet of metal and air heating/cooling would be cheaper but may be more difficult to control accurately. | |||
*[http://www.mcmaster.com/#standard-aluminum-sheets/=lfqnfz Aluminum bars from McMaster-Carr] | |||
:1 cm x 4 cm x 6 feet for $35 (enough for 45 4x4 cm tube holders) | |||
:1 cm x 4 cm x 6 feet for $44 (enough for 36 5x5 cm tube holders) | |||
:1 cm x 2.5 cm x 6 feet for $23 | |||
= Previous work = | |||
= Existing DIY cyclers = | == Existing DIY cyclers == | ||
*[http://openpcr.org/ openpcr] | *[http://openpcr.org/ openpcr] | ||
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*griessen pcr | *griessen pcr | ||
*[http://pimm.wordpress.com/2009/10/31/lavaamp-cheapest-pocket-pcr-thermocycler-dreamed-for-diy-biologists/ lava-amp] | *[http://pimm.wordpress.com/2009/10/31/lavaamp-cheapest-pocket-pcr-thermocycler-dreamed-for-diy-biologists/ lava-amp] | ||
*[http://diyhpl.us/~bryan/papers2/microfluidics/2010-01-20/Petri%20dish%20PCR%20-%20laser-heated%20reactions%20in%20nanoliter%20droplet%20arrays.pdf laser-heated petri dish PCR] | *[http://diyhpl.us/~bryan/papers2/microfluidics/2010-01-20/Petri%20dish%20PCR%20-%20laser-heated%20reactions%20in%20nanoliter%20droplet%20arrays.pdf laser-heated petri dish PCR] | ||
*[http://144.206.159.178/ft/943/73743/1264824.pdf A portable battery-operated chip thermocycler based on induction heating] | |||
== Useful links == | |||
* [http://openwetware.org/wiki/DIYbio:Notebook/Open_Thermal_Cycler DIYbio:Notebook/Open Thermal Cycler] | |||
* [http://russelldurrett.com/lightbulbpcr.html The Light Bulb PCR Machine] | |||
* [http://groups.google.com/d/topic/diybio/XqIJ3l-IHZ8/discussion $10 buck thermocycler design work] | |||
* [http://groups.google.com/d/msg/diybio/r7opEnSApkU/arffsaDtv_oJ Re: inexpensive PCR designs UnBlockedâ„¢] | |||
* [http://groups.google.com/d/topic/diybio/ZzMZohMpgB8/discussion The open-source Pacer PCR] | |||
* [http://groups.google.com/d/topic/diybio/tlPUBoCTf-Q/discussion Cheaper OpenPCR] | |||