Biohackers February 2013

biohackers@sudoroom.org

6 participants
4 discussions

by Marc Juul

Does anyone have a pressure cooker they can donate to sudo room? We need one for the following projects: * open bio lab :For general sterilization of tubes, pipette tips, glassware, etc. * sudoshroom - mushroom growing :For sterilizing growth medium * subiir - the sudo beer :For sterilizing glycerol for glycerol stock of beer yeast. Means we don't have to buy yeast every time. -- Marc Juul

11 years, 2 months

Re: [Biohackers] [sudo-discuss] Name ideas for the Open Source Desktop Bioengineering lab?

by Marc Juul

On Thu, Feb 14, 2013 at 7:50 AM, Ryan Bethencourt <ryan.bethencourt(a)gmail.com> wrote: > Hi All, Hey Ryan. Replying to biohackers list as well (since we should use that one). > Yesterday a few of you brought up a great point, the "Open Source Desktop > Bioengineering lab" is a bit of a mouthful for a project name. Do any of you > have creative ideas for a shorter, snappier name? > > I thought it made sense to open source the name just like we're open > sourcing the project :) You mean open source the naming, not the name right? I suggested: Lab on a desk. Or we could call it the Molecular Lab On A Desk (The MoLoad). -- Marc Juul

11 years, 2 months

PCR machine

by Marc Juul

I've done some work on the PCR machine I've focused on making a design that can both be realistically built by off-the-shelf components by anyone with a basic set of skills, and is still cheap to make when we scale up to production of 100x to 500x. I think we may be able to realistically sell the whole lab kit for $400. We shouldn't spend more than half of that amount on components (including chemicals/biologicals/etc). The rest should go to pay for labor. We have four devices we've been talking about: *PCR machine *Centrifuge *Gel electrophoresis device with transilluminator *Incubator with shaker So if we spend e.g. $40 on components per piece of lab equipment on average, that's $160, leaving $40 for chemicals/biologicals/pcr tubes and other consumables. I think we should include enough stuff for the first 2-3 experiments, including nice manuals for everything. Hopefully the labor costs will be relatively low since we'll be selling this in kit form (a lot of assembly required). One problem is that we're not including a micropipette. We could include some simple glass pipettes and recommend that people go on ebay and buy a couple of better pipettes, or we could try to buy some chinese knockoffs or buy up a bunch of used pipettes and sell two pipettes for e.g. an extra $100. I've put all of the info here: http://sudoroom.org/wiki/Open_Biotech/PCR Ryan has ordered the components needed to build the first prototype, so we should be able to start building next week. One thing I haven't looked at yet is a suitable case. We may want to look at DIY vacuum-formed biodegradable plastic sheets for the PCR machine and gel box, and a bolted/riveted box of metal for the centrifuge. For the incubator it's obvious that polystyrene can't be beaten for price/performance, but I think we should look at more biodegradable materials. I've been talking to Jake (from Noisebridge) and he will help us design a cheap and safe power supply for gel electrophoresis. -- Marc Juul

11 years, 2 months

Re: [Biohackers] [sudo-discuss] Name ideas for the Open Source Desktop Bioengineering lab?

by Marc Juul

> Is there a Biolabhackerdesktopproject meeting tonight? Well. I'm here, but no-one else has showed up so far. -- Marc Juul

11 years, 2 months

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Biohackers February 2013