27 Sep
2013
27 Sep
'13
11:23 a.m.
Harbor Freight (mostly chinese-imported tool store across USA) sells
dremel-like tools for $9 with accessories, not to mention the always
available %20 off coupons on retailmenot.com
On Thu, Sep 26, 2013 at 11:14 AM, Matthew Harbowy <hbergeronx(a)gmail.com> wrote:
In most cases, a 2000g, 6000 rpm minifuge is all you
need for mini preps and
pelleting DNA. Galaxy VWR minifuge for this is $422, brand new, from VWR.
This kit is $260. You can pick up minifuges at used resellers for <$50. A
basic Dremel tool is about $50. A replacement rotor for the galaxy microfuge
(hint, hint) is $70.
To Patrik's point: how much is your eyesight worth? Be careful out there.
When making plastic covers, use highly resistant materials, like
polycarbonate laminates (e.g. MAKROLON). Metal rotors are probably a better
idea than plastic ones. IMO, the brand new minifuge is not so expensive and
has clearly traceable product liability. DIY in this case might be a great
learning tool, if by "losing an eye" you learn what not to do.
This has been your "Important safety tip there, Egan" of the day.
Matt
On Thursday, September 26, 2013, Patrik D'haeseleer wrote:
--
-Nathan
I would also want to see what happens to that thin acrylic cover when a
fully laden tube ejects from the rotor at full speed. High speed centrifuges
are explosion-prone devices, and need to be tested as such. Flying shards of
acrylic are no fun...
The typical recommendation for using the dremelfuge are to hold it inside
a metal cooking pot - at least that will give you some serious protection.
Patrik
On Thu, Sep 26, 2013 at 10:17 AM, Craig Rouskey <craigrouskey(a)gmail.com>
wrote:
--
-- You received this message because you are subscribed to the Google Groups
DIYbio group. To post to this group, send email to diybio(a)googlegroups.com.
To unsubscribe from this group, send email to
diybio+unsubscribe(a)googlegroups.com. For more options, visit this group at
https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups
"DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an
email to diybio+unsubscribe(a)googlegroups.com.
To post to this group, send email to diybio(a)googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit
https://groups.google.com/d/msgid/diybio/CA%2BRBL9k8bVp%3Doguh5t4Ea%3DM2wZJ….
For more options, visit https://groups.google.com/groups/opt_out.
Thanks Jenny! Typically for DNA work centrifuge speeds need to be approx.
13000rpm/18000xg
—
Craig J. Rouskey
contact(a)craigrouskey.com
http://www.craigrouskey.com
On Thu, Sep 26, 2013 at 1:15 AM, Jenny Ryan <tunabananas(a)gmail.com>
wrote:
Via @BiologiGaragen: New open source centrifuge
http://t.co/otDqV44UER http://t.co/54l7f5DNOt
Shared via TweetCaster
_______________________________________________
Biohackers mailing list
Biohackers(a)lists.sudoroom.org
http://lists.sudoroom.org/listinfo/biohackers