Hey all, Here is the video on DIY Molecular Design & Cloning by Craig that was held by CounterCultureLabs at sudoroom on 1/14/14. Video can be found here: http://www.youtube.com/watch?v=kKiqh-FhyNo Lecture details a basic overview of designing and cloning microorganisms using open access databases. Subjects discussed include: - Primer design -- tricks, tools and resources - PCR-based cloning -- methods and means for the DIYer - Purification, Isolation and Verification - Databases and software used for design and cloning Join us for part II of this lecture series on cloning. Craig Rouskey will talk in depth on PCR: a. PCR Principle b. Components/role of each component c. setting up PCR/Making adjustments to reagents d. making PCR buffers (Maniatis) e. Reconstituting Primers f. Working the thermocycler Sign up for the event here: http://www.meetup.com/Counter- Culture-Labs/events/161499622/ Held on 2/11/14 6:30PM @ sudoroom For more CounterCultureLabs events visit the meet up schedule @ http://www.meetup.com/Counter-Culture-Labs/ Happy sciencing, Louis -- You received this message because you are subscribed to the Google Groups "Counter Culture Labs" group. To unsubscribe from this group and stop receiving emails from it, send an email to counterculturelabs+unsubscribe(a)googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out.