[sudo-discuss] CCL: Craig's DIY Molecular Design & Cloning video

Hey all, Here is the video on DIY Molecular Design & Cloning by Craig that was held by CounterCultureLabs at sudoroom on 1/14/14. Video can be found here: http://www.youtube.com/watch?v=kKiqh-FhyNo Lecture details a basic overview of designing and cloning microorganisms using open access databases. Subjects discussed include: - Primer design -- tricks, tools and resources - PCR-based cloning -- methods and means for the DIYer - Purification, Isolation and Verification - Databases and software used for design and cloning Join us for part II of this lecture series on cloning. Craig Rouskey will talk in depth on PCR: a. PCR Principle b. Components/role of each component c. setting up PCR/Making adjustments to reagents d. making PCR buffers (Maniatis) e. Reconstituting Primers f. Working the thermocycler Sign up for the event here: http://www.meetup.com/Counter-Culture-Labs/events/161499622/ Held on 2/11/14 6:30PM @ sudoroom For more CounterCultureLabs events visit the meet up schedule @ http://www.meetup.com/Counter-Culture-Labs/ Happy sciencing, Louis