I have a potentially prize winning idea, but I need your help...
As you might know, I've been teaching Agar Art workshops at CCL - painting
with colored bacteria! Next session is this coming Monday
<https://www.meetup.com/Counter-Culture-Labs/events/264878488/>. The
American Society for Microbiology is holding a yearly Agar Art Contest
<https://www.asm.org/Events/2019-ASM-Agar-Art-Contest/Home> that we can
submit some of our best work to.
My idea is to embed a little LED circuit *in the agar* in a Petri plate,
light up a UV LED, and then trace the circuit on top of the agar in
fluorescent E. coli!
Here's the part I need your help with... we'll need to experiment a bit
with different circuit implementations to figure out what works best. I can
easily solder a little "throwie" circuit myself, but I don't think I'll
have time to go through half a dozen designs to see what works best (or at
all). So ideally I'd love to partner up with somebody why can crank out a
couple circuit versions while I focus on the wetlab side. You'll get full
share of the credit and any prize money of course.
A couple complications:
- I would love to be able to autoclave the circuit before embedding it
in agar, to eliminate any possible contamination. This involves heating to
121C/250F for 20 minutes under steam. Not sure which materials will stand
up to that (batteries? plastic LED?)
- The normal E. coli growth medium is quite conductive. We should be
able to use a medium without salt, but it will likely still have some
conductivity. We could try coat the entire circuit in clear epoxy to
insulate it though.
- The circuit will still need to give off enough light after been
embedded in agar for several days. Coin cell throwies do last a couple
days, but UV LEDs are a bit more power hungry, so perhaps we could try a
AAA?
- Having the UV LED on continuously may not be healthy for the bacteria
growing on top, so we may need to include a Reed switch or some other
mechanism to turn the LED on remotely.
- More complicated circuits = extra awesomesauce! Can we build a circuit
that measures the pH in the agar under an acid producing bacteria? Create a
grid of electrodes that affects the growth of bacteria growing on top of it?
- Ideally, the whole circuit would fit inside a 10cm Petri dish - let's
call it 9.5cm diameter, and no more than about 1cm in height. If necessary,
we do have access to larger and deeper Petri dishes though.
Patrik
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Who's going and to which of the protests?
Looks like there's one at Laney but it's more of a group gathering
there and going together to the Youth vs. Apocalypse protest happening
in SF.
https://globalclimatestrike.net/
--
marc/juul
I've been talking about epoxying the Sudoroom floor all year, which
needs to happen during the dry season because humidity makes
everything take way longer. It just rained today and winter is coming
fast. Meanwhile I've had a couple big surprises in my personal life
that need attention.
So I'm admitting defeat. I can't do it this year. If nobody else wants
to lead the Great Sudo Rebase, I will try again in summer 2020.
Hi,
I am a newcomer and I'm really enjoying my participation. I am working on
setting up a computer for the Lulzbot 3D printer. With Jake's help I got a
fresh version of Mint installed and a recommended Lulzbot Cura software
package.
https://www.lulzbot.com/cura
Need some help solving errors in the Cura installation.
I've been coming Tuesday night and have availability for other times. Let
me know if you can help move the 3D printer forward.
Please email me directly as I don't have a login or password for your
lists.
Thanks
Ross
i have a box of old macbooks, some could be made to work, some work.. there are
also a couple of ancient macbook pros maybe... all Intel CPUs so yes they can
run linux. Most are 4GB RAM maximum though. I also have a ton of power
adaptors but again they need soldering to work.
free if you're not just going to throw them out, but you can't keep them at
sudoroom! must take them home.
https://sfbay.craigslist.org/eby/sop/d/oakland-bunch-of-old-macbooks/695536…
-jake
we really need to fix the north wall bottom plate & a large section of the
roof over sudoroom before the rainy season comes or we're going to have
leaks this winter
any volunteers?
:/
Hello Sudo Room, Adam & I worked on the back shop area, which is why the
tools and material is a bit messy. There is still a bit of work to do.
Please pardon the mess while it is being sorted and organized. I will be
back later this afternoon to continue sorting and organizing.
Thanks
Rayc
