Re: [sudo-discuss] [CCLmembers] Re: New OpenTrons OT-2 in Sudo/CCL!

Hey Patrik, I have a single p10 and a broken multichannel 300ul that I'll bring over (I bet you may be able to fix the multichannel 300ul). I'll also think of a good way to automate maceration of mushrooms. I feel like it may be a bit difficult due to cross-contamination issues. Keoni On Thu, Oct 4, 2018 at 3:58 AM Patrik D'haeseleer &lt;patrikd(a)gmail.com&gt; wrote: > Hi Keoni - do you have any pipettes for the OT-2? > > We installed it right on the boundary between CCL and Sudo for now. > Once we start doing real experiments, we should move it into the wetlab > though. > > We also happen to have a Biometra TRobot, which is essentially a > thermocycler with an automated lid, so it can be easily incorporated into a > liquid handling robot. That should give us an ideal platform for PCR > applications on the Opentrons... > > DNA barcoding of mushroom species is probably our #1 killer app for > this robot. When Alan Rockefeller comes back from his current outing > throughout Mexico, he will likely bring several hundred mushroom samples > with him, all of which will need to be submitted to PCR amplification and > Sanger sequencing. Alan usually mashes each sample with a little plastic > pestle in an Eppendorf tube - not sure how to replicate that on the robot, > but we could brainstorm a little device to macerate mushroom samples... > > Patrik > > On Sun, Sep 30, 2018 at 6:42 PM Keoni Gandall &lt;koeng101(a)gmail.com&gt; > wrote: > >> The hardware issue should be easily solved with a little hack that >> deactivates OpenTrons pipette-verification (it worked before a certain >> software update). >> >> The hope is that the robot can help bridge the gap between the >> software folks at SudoRoom and wetware folks in CCL. >> >> I'll at hands on workshop as well - come find me in the red polo if >> you'd like to talk about the machine! >> >> On Sun, Sep 30, 2018 at 6:34 PM Patrik D'haeseleer &lt;patrikd(a)gmail.com&gt; >> wrote: >> >>> Hi all, >>> >>> You may have noticed that Keoni Gandall dropped of a brand new (ish) OpenTrons >>> OT-2 liquid handling robot <http://opentrons.com/ot-2>recently! This >>> is the much smaller and newer sibling to the giant 5'x5' robot CCL has in >>> the basement. It is currently sitting in SudoRoom, and may or may not have >>> a hardware or electrical issue to be diagnosed first. >>> >>> Also, if you'd like to learn how to use this machine, OpenTrons is >>> running a *FREE HANDS-ON WORKSHOP* to learn how to use the OT-2 >>> tomorrow at the Mission Bay Conference Center; Monday 2-4pm. Registration >>> is free, but space may run out, so I would recommend you sign up asap: >>> >>> >>> https://www.eventbrite.com/e/opentrons-hands-on-workshop-tickets-49398612580 >>> >>> Patrik >>> >> --