Hi Sudo Room,
CCL is looking to donate our large liquid handling robot in order to free
up some space in the lab. Would you or anyone you know like it? It's
important that any organization that CCL transfers the robot to is also a
501(c)(3).
Here are some specifics about the robot:
Capabilities:
- Liquid handling
- Freezer with automated door
- With a little TLC, could carry out many basic biology assays and
operations, like plate incubation, PCR, SDS-PAGE, and centrifugation.
The robot includes:
- Robot frame made of 80/20 aluminum bars
- A gantry with a pipette mounted to it that can move in 3-dimensions, plus
actuate the pipette aspirator. All dimensions have limit switches on them
- A Smoothieboard <http://smoothieware.org/smoothieboard> to run the
3D-movement motors
- An Arduino Uno to manage electrical relays
- A Raspberry Pi 2 that can be used to communicate to the Smoothieboard and
Arduino
- A top-loading freezer for storing samples. Parts included to automate
opening and closing the freezer.
- Custom-built table with deckspace for robot operation and storage space
underneath
- Various pieces of labware, including a microcentrifuge and custom-cut
acrylic to allow the robot to manipulate petri plates
State:
- Robot moves in 3 dimensions. The motor for the pipette jogger has been
shown to work, but is currently not connected
- All of the other, non-movement components are included but not assembled
- No software provided, but we can definitely share the documents we've
generated as we learned how to use it
I've attached a few pictures and a short video
<https://photos.app.goo.gl/GhuH4GS2qNGuR58M9> of the robot picking up a
pipette tip and ejecting it to help get an idea of the way it operates.
That said, the robot is very hacker-friendly, so alternative uses are
doable.
If you've got any leads, reach out to me, the CCL Board, or the CCL Space
Committee. Hoping to find a good home for this robot!
-Tim
Dear Sudo Room,
Here's your invoice! We appreciate your prompt payment.
Thank you for stewarding the commons.
Love and solidarity,
Omni Commons
------------------------ Pledge Summary --------------------------
Invoice # : 2035
Invoice Date: 03/01/2020
Due Date: 03/25/2020
Terms: Net 25
Amount Due: $4,000.00
The complete version has been provided as an attachment to this email.
---------------------------------------------------------------------
Thanks everyone for your efforts so far!
*Remaining jobs to finish by early Thursday morning, in vague priority
order, and probably not exhaustive*
* Shattuck and 48th st doors - adjust panic bars and door hardware so
they open easily
* Remove or safely store fluorescent tubes that are in corner of EH
* Put a gas shutoff wrench by gas meter if there isn’t one
* Safely store boxes of batteries in sudo
* Install smoke detectors (in locked storage)
* 48th st hallway - rehang fire extinguisher, paint and install
baseboards and or molding if we have, remove blue tape, final mop,
leave completely clear (bonus points if you can mop/scrub the
hallway to the bocce court with TSP while you're at it)
* Matt, Monk, electrical crew - fisnish mapping electrical system,
covering open panels, emergency lighting and exit signs installed
and functional, wiring, replacing eh fixtures, etc
* *BASEMENT - organize onto shelves or get rid of all stuff in piles;
Make basement look like storage, not event space; store Ken finish
removing stuff, organize printers, remove all shiny stuff from
ceiling, remove and store all extension cords *
* Finish painting wooden windows
* Remove/paint over small amount of graffiti on outside of building
towards 48th st doors
* *Finish and install awning
*
* Move stove remnants, other metal from basement kitchen to EH for
metal waste pickup on Wednesday
* Bocce court bathroom - clean and organized, w compost and trash cans
* 48th st hallway - *rehang fire extinguisher*, paint and install
baseboards and or molding if we have, final mop, leave completely clear
* Stage left bathroom -*paint stained side of ceiling w glossy white*,
also stalls. remove graffiti on mirror, general cleaning
* Disco room - *finish painting*, *clean plexi floor*, remove mirrors
that aren’t hung to dump or store safely, restick Marley (tape in
cabinet is disco room - Luka watched video on how to lay properly.
If there isn't time for this just layit out flat over the floor )
* Small classroom- remove excess chairs, boxes in front of electrical
panel, cobwebs, straighten out
* Bathrooms cleaned
* Ballroom bar - final sweep and organize
* Trash room - should look organized w minimum stacked cardboard
* Make sure Shower is not dripping
* Entry area to bocce court organized
* Get rid of all trash piled in sudo
* Basement northwest corner (where Village stuff was) ceiling water
damage - cover and potentially replace damaged ceiling tiles
* Remove hanging wires in den. If time, prime and paint ceiling
* Sudo mesh area organized
* Move Stage section upstairs
* Fix broken stair steps
* Disco room door - Wash w tsp; if time, prime and paint w grey in the
storage
* *Wednesday evening - fisnish up any work in progress, general
cleaning and organizing so everything is in its place, organize
trash cans in rooms (a trash, recycling and compost can grouped
together, except bathrooms need one compost and one trash) and put
in bags/liners *
*
*
Remaining inspection prep jobs
Power wash exterior Inc wooden window sills
Sand, patch, paint wooden window sills
Replace broken window in eh
Replace missing window skate (ace hardware on grand has them per mike Tenuto)
48th st hallway - finish priming, paint, baseboards, remove blue tape in ceiling, molding if time, final mop
Matt, Monk, electrical crew - mapping electrical system, covering open panels, wiring, replacing eh fixtures, etc
Remove piles of stuff remaining in basement
Set up printers in media lab - at least organize so they look like they are functional
Basement Couches upstairs - black one to dump
Move Stage section upstairs
Fix broken stair steps
Paint white wall in eh mezz w eh white - all prep done
Disco room - finish painting, clean Marley, clean plexi floor, restick Marley ( tape arrived yesterday ). Lib lens said they would take on a lot of this
Eh mezz bathroom finish cleaning walls and baseboards w tsp, rinse w water, prime and paint w gloss white. Cover all graffiti
Clear out/clean shelves next to fridge in eh mezz
Den printer to dump pile
Move stove remnants, other metal from basement kitchen to eh.
Clean out trash room - everything except trash bins to dump pile
Rent large truck for dump runs on Monday w possibility of follow up trips on tuesday
Put a gas shutoff wrench by gas meter if there isn’t one
Was new water heater installed w earthquake bracing?
WEDNESDAY - general cleaning, finish jobs
Other details here: https://docs.google.com/document/d/1XJ6HRW32KeqP9ZCWXjCvXPqoNmHmyt4dIR41EZN…
Sent from my iPhone
Hi everyone,
After I asked our insurance agent why our property insurance will be
going up $800 this year, she sent the following reply from the company:
/On a side note, it appears the condition of this building has
drastically deteriorated over the years. The current condition of the
building is below average and is unacceptable. We will remain on this
account for one more year but please be advised that we will be setting
this account up for nonrenewal. If the insured is able to provide proof
of building updates and proper maintenance, we may be able to review
this account again for eligibility. In additional, updates would allow
us to approach other carriers for alternate quotes./
*If we lose our property insurance and are unable to find another
company (unlikely and, if we can, the rate will be high), we will
probably not be able to refinance and we will lose the building. *
**
Our agent thinks they are basing their evaluation of the building
condition only upon seeing the exterior, possibly on google street view.
That is why they have asked to do the inspection that I have already
told you about. I think that will happen on Thursday 2/13
We need all hands on deck over the next 2 weeks to make the building
look as good as possible, inside and out. That means:
- fire safety equipment all in working order, NO clutter, no fire hazards
- condition of any wiring as good as possible
- exterior condition as good as possible, no graffiti, clean, EH windows
fixed and window sills repaired and painted, new awning up
- any roof trouble spots fixed
- general interior condition should look as good and safe as possible,
general painting where needed, everything clean and in working order
- fixing anything that could be seen as a safety hazard.
I propose an emergency meeting tomorrow evening at 7pm to get organized
to tackle these things.
Laura
We then translated the aligned genome and found that these inserts are present
in all Wuhan 2019-nCoV viruses except the 2019-nCoV virus of Bat as a host
[Fig.S4]. Intrigued by the 4 highly conserved inserts unique to 2019-nCoV we
wanted to understand their origin. For this purpose, we used the 2019-nCoV
local alignment with each insert as query against all virus genomes and
considered hits with 100% sequence coverage.
Surprisingly, each of the four inserts aligned with short segments of the Human
Immunodeficiency Virus-1 (HIV-1) proteins. The amino acid positions of the
inserts in 2019-nCoV and the corresponding residues in HIV-1 gp120 and HIV-1
Gag are shown in Table 1. The first 3 inserts (insert 1,2 and 3) aligned to
short segments of amino acid residues in HIV-1 gp120. The insert 4 aligned to
HIV-1 Gag. The insert 1 (6 amino acid residues) and insert 2 (6 amino acid
residues) in the spike glycoprotein of 2019-nCoV are 100% identical to the
residues mapped to HIV-1 gp120. The insert 3 (12 amino acid residues) in 2019-
nCoV maps to HIV-1 gp120 with gaps [see Table 1]. The insert 4 (8 amino acid
residues) maps to HIV-1 Gag with gaps.
Although, the 4 inserts represent discontiguous short stretches of amino acids
in spike glycoprotein of 2019-nCoV, the fact that all three of them share amino
acid identity or similarity with HIV-1 gp120 and HIV-1 Gag (among all annotated
virus proteins) suggests that this is not a random fortuitous finding. In other
words, one may sporadically expect a fortuitous match for a stretch of 6-12
contiguous amino acid residues in an unrelated protein. However, it is
unlikely that all 4 inserts in the 2019-nCoV spike glycoprotein fortuitously
match with 2 key structural proteins of an unrelated virus (HIV-1).
https://www.biorxiv.org/content/10.1101/2020.01.30.927871v1.full.pdf
