4 Oct
2018
4 Oct
'18
3:58 a.m.
Hi Keoni - do you have any pipettes for the OT-2?
We installed it right on the boundary between CCL and Sudo for now. Once we
start doing real experiments, we should move it into the wetlab though.
We also happen to have a Biometra TRobot, which is essentially a
thermocycler with an automated lid, so it can be easily incorporated into a
liquid handling robot. That should give us an ideal platform for PCR
applications on the Opentrons...
DNA barcoding of mushroom species is probably our #1 killer app for this
robot. When Alan Rockefeller comes back from his current outing throughout
Mexico, he will likely bring several hundred mushroom samples with him, all
of which will need to be submitted to PCR amplification and Sanger
sequencing. Alan usually mashes each sample with a little plastic pestle in
an Eppendorf tube - not sure how to replicate that on the robot, but we
could brainstorm a little device to macerate mushroom samples...
Patrik
On Sun, Sep 30, 2018 at 6:42 PM Keoni Gandall <koeng101(a)gmail.com> wrote:
The hardware issue should be easily solved with a
little hack that
deactivates OpenTrons pipette-verification (it worked before a certain
software update).
The hope is that the robot can help bridge the gap between the software
folks at SudoRoom and wetware folks in CCL.
I'll at hands on workshop as well - come find me in the red polo if you'd
like to talk about the machine!
On Sun, Sep 30, 2018 at 6:34 PM Patrik D'haeseleer <patrikd(a)gmail.com>
wrote:
Hi all,
You may have noticed that Keoni Gandall dropped of a brand new (ish) OpenTrons
OT-2 liquid handling robot <http://opentrons.com/ot-2>recently! This is
the much smaller and newer sibling to the giant 5'x5' robot CCL has in the
basement. It is currently sitting in SudoRoom, and may or may not have a
hardware or electrical issue to be diagnosed first.
Also, if you'd like to learn how to use this machine, OpenTrons is
running a *FREE HANDS-ON WORKSHOP* to learn how to use the OT-2 tomorrow
at the Mission Bay Conference Center; Monday 2-4pm. Registration is free,
but space may run out, so I would recommend you sign up asap:
https://www.eventbrite.com/e/opentrons-hands-on-workshop-tickets-49398612580
Patrik