Hi All,
I'm sending this invite out on behalf of Nina's Idealab journal club who
will be leading the journal club with Derek tonight to discuss Gene editing
technology, please feel free to join and also sign up for the free virtual
meet up!
Gene editing technology ROCKS :)
http://www.meetup.com/Berkeley-Biolabs/events/136129042/
The next global Biojournal club is this Thursday, Aug 22nd at 5:30pm PST.
The session will be held via zoom.us. If you are interested in
participating, please …
[View More]make a zoom.us account if you have not done so
already.
We can also send you 2 short papers on CRISPR applications. Derek Jacoby
will discuss one of them, and Nina will go through the other.
Nina DiPrimo, PostDoc at UC Berkeley, Biocoder Journal editor and Berkeley
Biolabs Founding Partner will be leading the discussion and will be the
primary contact if you have any questions.
More info on CRISPR's from Wikipedia:
*CRISPRs* (*C*lustered *R*egularly *I*nterspaced *S*hort*P*alindromic
*R*epeats)
are loci <http://en.wikipedia.org/wiki/Locus_(genetics)> containing
multiple short direct repeats that are found in the genomes of
approximately 40% of sequenced
bacteria<http://en.wikipedia.org/wiki/Bacteria> and
90% of sequenced archaea <http://en.wikipedia.org/wiki/Archaea>. CRISPR
functions as a prokaryotic immune system, in that it confers resistance to
exogenous genetic elements such
asplasmids<http://en.wikipedia.org/wiki/Plasmids>
and phages <http://en.wikipedia.org/wiki/Phage>.The CRISPR system provides
a form of acquired immunity <http://en.wikipedia.org/wiki/Acquired_immunity>.
Short segments of foreign DNA, called spacers, are incorporated into the
genome between CRISPR repeats, and serve as a 'memory' of past
exposures. CRISPR spacers are then used to recognize and silence exogenous
genetic elements in a manner analogous to
RNAi<http://en.wikipedia.org/wiki/RNAi> in
eukaryotic organisms.
--
Ryan Bethencourt
Tel: (415) 825 2705
Conf Call: (650) 741 5013
ryan.bethencourt(a)gmail.com
http://www.litmususa.com/http://berkeleybiolabs.com/www.bamh1.comwww.linkedin.com/in/bethencourtwww.logos-press.com/books/biotechnology_business_development.php
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i'm sorry, my last post on this subject came out wrong. Mike Kan of all
people pointed out that it was a huge list of novels!
I included the complete list of files as four attached textfiles, which
were turned into raw text by the mailing list. The list of novels drowned
out everything else, which was much more valuable information.
here is a much more useful list of what's on there (ignoring the kindle
folder and the Books folder)
this is all on the 4TB external hard drive currently …
[View More]on the main table at
sudoroom.
oh also, we will now have 10x traffic from the interweb because of all the
book titles posted in cleartext. maybe people will visit sudoroom to read
the files off the disk?
-jake
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I think part of the problem with that is that if you want to make your
openPCR do the job in a reasonable time frame, you need to be able to
thermally cycle your fluids rapidly.
every bit of material you have that is thermally connected to the PCR
vials and heating/cooling apparatus is thermal mass that you need to
swing, which makes your energy demand greater on your heat pump, and/or
slows down your PCR time.
that's why the best material for touching the vials will likely be a thin
…
[View More]skin of aluminum, shaped like inverted cones on one side and flat surfaces
on the other side, but mostly hollow. Such a shape would be designed in
openSCAD, modeled with a 3D printout, and either cast at OMI or Jim's or
Bernards, or more likely ordered as a metal print from Pomoko or Shapeways
or something.
unless you don't care about PCR time, in which case yes, you can make your
own thermal manifold out of a block of aluminum or lead.
-jake
Aug 21, 2013 01:58:57 PM, patrikd at gmail.com wrote:
> Hi Hol,
>
> Why don't you guys use Hackteria's drill bit to drill the wells in the
> aluminum heat block? They designed and ordered a custom bit specifically
> to match the 17.5 degree cone angle used in OpenPCR, and they're willing
> to let other groups borrow it. Perfect example of leveraging the global
> DIY community!
>
> http://hackteria.org/wiki/index.php/Wild_OpenPCR
>
> Simon Field on the DIYbio list suggested a while back to cast the heat
> block from a lower-melt alloy. That's potentially something that anyone
> with a hot plate could achieve, and you could even use your own favorite
> PCR tubes to make a perfectly fitting mold.
>
>Patrik
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as you may have noticed, there is a telephone at sudoroom
it's a grey triangle that kinda looks like the klingon flag, and has an
on-off button on its keyboard. It's a corporate speakerphone.
last night we added a ringing bell to the phone line, so that when people
call it will make a proper noise.
when you hear the bell, press the on-off button on the speakerphone so you
can talk to whoever is calling sudoroom.
the phone number to sudoroom is 510-858-7145
if there is nobody at …
[View More]sudoroom, and someone calls, it doesn't matter that
the ringer is so loud because nobody lives at sudoroom or would sleep
there, so it shouldn't matter if there's a bell ringing in the middle of
the night and nobody answering it.
-jake
PS alternate plans are, replace the speakerphone with a payphone (so it's
more obvious how to answer it) or get Georgio to make a circuit to cause
the speakerphone to automatically pick up when the phone rings..
or we could just label the on-off button better.
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Dear All,
Lina from Tekla labs has reached out and would love to have some entries
for the DIY lab equipment contest from CCL and Sudo Room. If you're
interested please sign up :)
"Tekla Labs is about to launch a DIY Lab equipment contest, hosted by the
maker site Instructables. We'll go live this August (ie any day, as soon as
the final t's are crossed). There'll be a Lab Equipment category, as well
as a Lab Hacks option for quick and clever hardware solutions in the lab.
We'd love to get …
[View More]some help getting word out about the competition and maybe
see some Bay Area folks (and beyond) applying for the $5000 in prizes.
Even more immediately though, you and your DIY network could really help us
out by simply helping us create some site activity by joining our member
network (which only takes a minute! I promise! And there's no spam)
http://www.teklalabs.org/members/<http://www.linkedin.com/redir/redirect?url=http%3A%2F%2Fwww%2Eteklalabs%2Eo…>
Of course, it'd be awesome if you or others submit designs (more soon) or
get involved in the competition itself in some other capacity, but this
quick 1 minute favor would really help our group of fellow science
enthusiasts! Please help spread the word! Thanks,
Lina"
--
Ryan Bethencourt
Tel: (415) 825 2705
Conf Call: (650) 741 5013
ryan.bethencourt(a)gmail.com
http://www.litmususa.com/http://berkeleybiolabs.com/www.bamh1.comwww.linkedin.com/in/bethencourtwww.logos-press.com/books/biotechnology_business_development.php
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We really need to put pressure on these F*!&^#(&-ing A*!&^#(&-holes at the Boy Scouts of America. They really do not seem to care about the world we all inhabit, and need to be put in their place.
MAKE, Sparkfun, Adafruit are into pressuring the BSA. If we can get a bunch of hackerspaces to do the same, we will make an impact.
"no matter what happens, how we all react to a group like the BSA will define "us" fighting and losing is ok, but i think you need to fight it all …
[View More]the way… think of a world where there are hacker scouts, learning sharing and it would all start with a shared victory together over an old oppressive scared group trying to get in the way of the future." -- Phil Torrone, of Adafruit
Here are just a few places to add some well-placed comments:
>>>>>> Boy Scouts Google+ page:
>>>>>> https://plus.google.com/u/0/+BoyScoutsofAmerica/posts
>>>>>>
>>>>>> Boy Scouts Facebook page:
>>>>>> https://www.facebook.com/pages/Boy-Scouts-of-America/113441755297
>>>>>>
>>>>>> Bot Scouts on Twitter:
>>>>>> https://twitter.com/boyscouts
>>>>>>
>>>>>> Boy Scouts on YouTube:
>>>>>> http://www.youtube.com/bsa100years
>>>>>>
>>>>>> Email:
>>>>>> PR(a)scouting.org
>>>>>>
>>>>>> The official publication for the adult volunteers site:
>>>>>> http://blog.scoutingmagazine.org
>>>>>>
>>>>>> On Twitter:
>>>>>> http://www.twitter.com/scouting
>>>>>>
>>>>>> On Facebook:
>>>>>> http://www.facebook.com/scoutingmagazine
>>>>>>
>>>>>> Email:
>>>>>> scoutingmag(a)gmail.com
>>>>>>
>>>>>> Boy's Life Magazine:
>>>>>> http://boyslife.org/
>>>>>>
>>>>>> On Twitter:
>>>>>> https://twitter.com/BoysLife
>>>>>> Email:
>>>>>> boyslife.mag(a)scouting.org
Best,
Mitch.
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At our last general meeting we had people mention they were very
uncomfortable with pitching their idea/project/innovation to a VC and those
alike, so I thought how could I help in that area!
Here it is guys
I host post show for the largest pitch event in Silicon Valley which is
called SF New Tech (sfnewtech.com)
The event is a monthly event where startups from across the world come to
launch officially in SF. Well launch or re launch or re introduce.
I wanted to personally invite a few Sudoers …
[View More]to come and observe and get
tips and inspiration from great startups. Network,meet friends, or lookf
for a job (people are always hiring)
This month you will hear Prezi, Fiverr, BitBalloon,SaasMax,Situation
Assesment Global,and Twibfy
The event is Wed Aug 21( so I will not be at the general meeting) and I
hate to steal people from it but it will be a great opportunity to look,
watch,observe,network,and meet people in Tech ,if thats your thing. Tickets
are 25, but I do have a guest list and I can get you in under #ThatTechGirl
for a smile.
So if you want to go for free let me know
I will be on bart
Doors open at 530 for free tacos
Pitches start at 730- I estimate a wrap at 930p
Location is Mighty at 119 Utah
FULL INFO- Go to http://libertymadison.com/?p=433 <http://t.co/KGbyGhxNR1>
415.937.3785
*Fiverr rescheduled for next month, due to a family emergency
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StoryCorps <http://storycorps.org/stories-archive/> is about preserving the
stories of our lives. Have a friend or family member interview you, or
interview them.
They are coming to Oakland. Open time slots this Friday at Peralta
Hacienda (in Fruitvale):
10am-11am
2pm-3pm
3pm-4pm
Contact Dennis Rojas if you're interested in taking part.
Hey all sudoers!
Tonight's Sudo Kids' theme is Twist Ties! We will b making twist tie art,
jewelry and doing some pre-viz for a twist tie stop motion epic (Atom &
Iris have kicked around a Twist Tie treatment of Moby Dick or The Rhyme of
the Ancient Mariner). So bring your twist ties or pipe cleaners and let's
make it happen!
Does anyone have a stop motion program? Something with onion skinning?
See you tonight 5pm for the start of food and 6:00pm-ish for festivities.
Oh, and tonight is …
[View More]the P.O.S.S.E. party. So bring delish-ous-ness!
Nom nom nom,
Ray
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