we cannot use the pick-and-place machine until the cover has been rebuilt
or is on a sturdier table. I have tested opening the heavy wooded cover,
And the hinges for it that are bolted into the shelf table, are starting to
tear and giveaway. the cover is also lifting up particle board platform.
The machine is sitting on as well.
I highly recommend that we do not attempt to use the pick-and-place machine
until we have a solid stable environment for it. Instead of a shelf unit.
Thank you Robert.
I have a potentially prize winning idea, but I need your help...
As you might know, I've been teaching Agar Art workshops at CCL - painting
with colored bacteria! Next session is this coming Monday
<https://www.meetup.com/Counter-Culture-Labs/events/264878488/>. The
American Society for Microbiology is holding a yearly Agar Art Contest
<https://www.asm.org/Events/2019-ASM-Agar-Art-Contest/Home> that we can
submit some of our best work to.
My idea is to embed a little LED circuit *in the agar* in a Petri plate,
light up a UV LED, and then trace the circuit on top of the agar in
fluorescent E. coli!
Here's the part I need your help with... we'll need to experiment a bit
with different circuit implementations to figure out what works best. I can
easily solder a little "throwie" circuit myself, but I don't think I'll
have time to go through half a dozen designs to see what works best (or at
all). So ideally I'd love to partner up with somebody why can crank out a
couple circuit versions while I focus on the wetlab side. You'll get full
share of the credit and any prize money of course.
A couple complications:
- I would love to be able to autoclave the circuit before embedding it
in agar, to eliminate any possible contamination. This involves heating to
121C/250F for 20 minutes under steam. Not sure which materials will stand
up to that (batteries? plastic LED?)
- The normal E. coli growth medium is quite conductive. We should be
able to use a medium without salt, but it will likely still have some
conductivity. We could try coat the entire circuit in clear epoxy to
insulate it though.
- The circuit will still need to give off enough light after been
embedded in agar for several days. Coin cell throwies do last a couple
days, but UV LEDs are a bit more power hungry, so perhaps we could try a
AAA?
- Having the UV LED on continuously may not be healthy for the bacteria
growing on top, so we may need to include a Reed switch or some other
mechanism to turn the LED on remotely.
- More complicated circuits = extra awesomesauce! Can we build a circuit
that measures the pH in the agar under an acid producing bacteria? Create a
grid of electrodes that affects the growth of bacteria growing on top of it?
- Ideally, the whole circuit would fit inside a 10cm Petri dish - let's
call it 9.5cm diameter, and no more than about 1cm in height. If necessary,
we do have access to larger and deeper Petri dishes though.
Patrik
Dear Sudo Room,
Here's your invoice! We appreciate your prompt payment.
Thank you for stewarding the commons.
Love and solidarity,
Omni Commons
------------------------ Pledge Summary --------------------------
Invoice # : 1903
Invoice Date: 10/01/2019
Due Date: 10/25/2019
Terms: Net 25
Amount Due: $4,000.00
The complete version has been provided as an attachment to this email.
---------------------------------------------------------------------
Who's going and to which of the protests?
Looks like there's one at Laney but it's more of a group gathering
there and going together to the Youth vs. Apocalypse protest happening
in SF.
https://globalclimatestrike.net/
--
marc/juul
I've been talking about epoxying the Sudoroom floor all year, which
needs to happen during the dry season because humidity makes
everything take way longer. It just rained today and winter is coming
fast. Meanwhile I've had a couple big surprises in my personal life
that need attention.
So I'm admitting defeat. I can't do it this year. If nobody else wants
to lead the Great Sudo Rebase, I will try again in summer 2020.
Hi,
I am a newcomer and I'm really enjoying my participation. I am working on
setting up a computer for the Lulzbot 3D printer. With Jake's help I got a
fresh version of Mint installed and a recommended Lulzbot Cura software
package.
https://www.lulzbot.com/cura
Need some help solving errors in the Cura installation.
I've been coming Tuesday night and have availability for other times. Let
me know if you can help move the 3D printer forward.
Please email me directly as I don't have a login or password for your
lists.
Thanks
Ross
