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Tonight at 5.30pm - Idealab- Journal Club - Gene Editing wth CRISPR technology (virtual meeting)

by Ryan Bethencourt

Hi All, I'm sending this invite out on behalf of Nina's Idealab journal club who will be leading the journal club with Derek tonight to discuss Gene editing technology, please feel free to join and also sign up for the free virtual meet up! Gene editing technology ROCKS :) http://www.meetup.com/Berkeley-Biolabs/events/136129042/ The next global Biojournal club is this Thursday, Aug 22nd at 5:30pm PST. The session will be held via zoom.us. If you are interested in participating, please make a zoom.us account if you have not done so already. We can also send you 2 short papers on CRISPR applications. Derek Jacoby will discuss one of them, and Nina will go through the other. Nina DiPrimo, PostDoc at UC Berkeley, Biocoder Journal editor and Berkeley Biolabs Founding Partner will be leading the discussion and will be the primary contact if you have any questions. More info on CRISPR's from Wikipedia: *CRISPRs* (*C*lustered *R*egularly *I*nterspaced *S*hort*P*alindromic *R*epeats) are loci <http://en.wikipedia.org/wiki/Locus_(genetics)> containing multiple short direct repeats that are found in the genomes of approximately 40% of sequenced bacteria<http://en.wikipedia.org/wiki/Bacteria> and 90% of sequenced archaea <http://en.wikipedia.org/wiki/Archaea>. CRISPR functions as a prokaryotic immune system, in that it confers resistance to exogenous genetic elements such asplasmids<http://en.wikipedia.org/wiki/Plasmids> and phages <http://en.wikipedia.org/wiki/Phage>.The CRISPR system provides a form of acquired immunity <http://en.wikipedia.org/wiki/Acquired_immunity>. Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures. CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi<http://en.wikipedia.org/wiki/RNAi> in eukaryotic organisms. -- Ryan Bethencourt Tel: (415) 825 2705 Conf Call: (650) 741 5013 ryan.bethencourt(a)gmail.com http://www.litmususa.com/ http://berkeleybiolabs.com/ www.bamh1.com www.linkedin.com/in/bethencourt www.logos-press.com/books/biotechnology_business_development.php

13 years

4tb hard drive on shelf

by Jake

i'm sorry, my last post on this subject came out wrong. Mike Kan of all people pointed out that it was a huge list of novels! I included the complete list of files as four attached textfiles, which were turned into raw text by the mailing list. The list of novels drowned out everything else, which was much more valuable information. here is a much more useful list of what's on there (ignoring the kindle folder and the Books folder) this is all on the 4TB external hard drive currently on the main table at sudoroom. oh also, we will now have 10x traffic from the interweb because of all the book titles posted in cleartext. maybe people will visit sudoroom to read the files off the disk? -jake

13 years

Re: [sudo-discuss] [CCL] Tekla Labs is about to launch a DIY Lab equipment contest

by Jake

I think part of the problem with that is that if you want to make your openPCR do the job in a reasonable time frame, you need to be able to thermally cycle your fluids rapidly. every bit of material you have that is thermally connected to the PCR vials and heating/cooling apparatus is thermal mass that you need to swing, which makes your energy demand greater on your heat pump, and/or slows down your PCR time. that's why the best material for touching the vials will likely be a thin skin of aluminum, shaped like inverted cones on one side and flat surfaces on the other side, but mostly hollow. Such a shape would be designed in openSCAD, modeled with a 3D printout, and either cast at OMI or Jim's or Bernards, or more likely ordered as a metal print from Pomoko or Shapeways or something. unless you don't care about PCR time, in which case yes, you can make your own thermal manifold out of a block of aluminum or lead. -jake Aug 21, 2013 01:58:57 PM, patrikd at gmail.com wrote: > Hi Hol, > > Why don't you guys use Hackteria's drill bit to drill the wells in the > aluminum heat block? They designed and ordered a custom bit specifically > to match the 17.5 degree cone angle used in OpenPCR, and they're willing > to let other groups borrow it. Perfect example of leveraging the global > DIY community! > > http://hackteria.org/wiki/index.php/Wild_OpenPCR > > Simon Field on the DIYbio list suggested a while back to cast the heat > block from a lower-melt alloy. That's potentially something that anyone > with a hot plate could achieve, and you could even use your own favorite > PCR tubes to make a perfectly fitting mold. > >Patrik

13 years

phone at sudoroom

by Jake

as you may have noticed, there is a telephone at sudoroom it's a grey triangle that kinda looks like the klingon flag, and has an on-off button on its keyboard. It's a corporate speakerphone. last night we added a ringing bell to the phone line, so that when people call it will make a proper noise. when you hear the bell, press the on-off button on the speakerphone so you can talk to whoever is calling sudoroom. the phone number to sudoroom is 510-858-7145 if there is nobody at sudoroom, and someone calls, it doesn't matter that the ringer is so loud because nobody lives at sudoroom or would sleep there, so it shouldn't matter if there's a bell ringing in the middle of the night and nobody answering it. -jake PS alternate plans are, replace the speakerphone with a payphone (so it's more obvious how to answer it) or get Georgio to make a circuit to cause the speakerphone to automatically pick up when the phone rings.. or we could just label the on-off button better.

13 years

Tekla Labs is about to launch a DIY Lab equipment contest

by Ryan Bethencourt

Dear All, Lina from Tekla labs has reached out and would love to have some entries for the DIY lab equipment contest from CCL and Sudo Room. If you're interested please sign up :) "Tekla Labs is about to launch a DIY Lab equipment contest, hosted by the maker site Instructables. We'll go live this August (ie any day, as soon as the final t's are crossed). There'll be a Lab Equipment category, as well as a Lab Hacks option for quick and clever hardware solutions in the lab. We'd love to get some help getting word out about the competition and maybe see some Bay Area folks (and beyond) applying for the $5000 in prizes. Even more immediately though, you and your DIY network could really help us out by simply helping us create some site activity by joining our member network (which only takes a minute! I promise! And there's no spam) http://www.teklalabs.org/members/<http://www.linkedin.com/redir/redirect?url=http%3A%2F%2Fwww%2Eteklalabs%2Eo…> Of course, it'd be awesome if you or others submit designs (more soon) or get involved in the competition itself in some other capacity, but this quick 1 minute favor would really help our group of fellow science enthusiasts! Please help spread the word! Thanks, Lina" -- Ryan Bethencourt Tel: (415) 825 2705 Conf Call: (650) 741 5013 ryan.bethencourt(a)gmail.com http://www.litmususa.com/ http://berkeleybiolabs.com/ www.bamh1.com www.linkedin.com/in/bethencourt www.logos-press.com/books/biotechnology_business_development.php

13 years

Boyscouts suing Hacker Scouts !

by Mitch Altman

We really need to put pressure on these F*!&^#(&-ing A*!&^#(&-holes at the Boy Scouts of America. They really do not seem to care about the world we all inhabit, and need to be put in their place. MAKE, Sparkfun, Adafruit are into pressuring the BSA. If we can get a bunch of hackerspaces to do the same, we will make an impact. "no matter what happens, how we all react to a group like the BSA will define "us" fighting and losing is ok, but i think you need to fight it all the way… think of a world where there are hacker scouts, learning sharing and it would all start with a shared victory together over an old oppressive scared group trying to get in the way of the future." -- Phil Torrone, of Adafruit Here are just a few places to add some well-placed comments: >>>>>> Boy Scouts Google+ page: >>>>>> https://plus.google.com/u/0/+BoyScoutsofAmerica/posts >>>>>> >>>>>> Boy Scouts Facebook page: >>>>>> https://www.facebook.com/pages/Boy-Scouts-of-America/113441755297 >>>>>> >>>>>> Bot Scouts on Twitter: >>>>>> https://twitter.com/boyscouts >>>>>> >>>>>> Boy Scouts on YouTube: >>>>>> http://www.youtube.com/bsa100years >>>>>> >>>>>> Email: >>>>>> PR(a)scouting.org >>>>>> >>>>>> The official publication for the adult volunteers site: >>>>>> http://blog.scoutingmagazine.org >>>>>> >>>>>> On Twitter: >>>>>> http://www.twitter.com/scouting >>>>>> >>>>>> On Facebook: >>>>>> http://www.facebook.com/scoutingmagazine >>>>>> >>>>>> Email: >>>>>> scoutingmag(a)gmail.com >>>>>> >>>>>> Boy's Life Magazine: >>>>>> http://boyslife.org/ >>>>>> >>>>>> On Twitter: >>>>>> https://twitter.com/BoysLife >>>>>> Email: >>>>>> boyslife.mag(a)scouting.org Best, Mitch.

13 years

TONIGHT- FREE TICKETS - LET ME KNOW ASAP

by Liberty Madison

At our last general meeting we had people mention they were very uncomfortable with pitching their idea/project/innovation to a VC and those alike, so I thought how could I help in that area! Here it is guys I host post show for the largest pitch event in Silicon Valley which is called SF New Tech (sfnewtech.com) The event is a monthly event where startups from across the world come to launch officially in SF. Well launch or re launch or re introduce. I wanted to personally invite a few Sudoers to come and observe and get tips and inspiration from great startups. Network,meet friends, or lookf for a job (people are always hiring) This month you will hear Prezi, Fiverr, BitBalloon,SaasMax,Situation Assesment Global,and Twibfy The event is Wed Aug 21( so I will not be at the general meeting) and I hate to steal people from it but it will be a great opportunity to look, watch,observe,network,and meet people in Tech ,if thats your thing. Tickets are 25, but I do have a guest list and I can get you in under #ThatTechGirl for a smile. So if you want to go for free let me know I will be on bart Doors open at 530 for free tacos Pitches start at 730- I estimate a wrap at 930p Location is Mighty at 119 Utah FULL INFO- Go to http://libertymadison.com/?p=433 <http://t.co/KGbyGhxNR1> 415.937.3785 *Fiverr rescheduled for next month, due to a family emergency

13 years

StoryCorps @ Peralta Hacienda this Friday

by Julio Rios

StoryCorps <http://storycorps.org/stories-archive/> is about preserving the stories of our lives. Have a friend or family member interview you, or interview them. They are coming to Oakland. Open time slots this Friday at Peralta Hacienda (in Fruitvale): 10am-11am 2pm-3pm 3pm-4pm Contact Dennis Rojas if you're interested in taking part.

13 years

East Bay Vegan Drinks this Thursday - 7pm Olde Depot

by Ryan Bethencourt

Hi All, Just in case you were interested, East Bay Vegan Drinks is this thursday at Old Depot (7pm) and all Sudo'ers and CCL'ers are welcome to join :) Here's the FB invite: https://www.facebook.com/events/179448848895514/ All the best, Ryan -- Ryan Bethencourt Tel: (415) 825 2705 Conf Call: (650) 741 5013 ryan.bethencourt(a)gmail.com http://www.litmususa.com/ http://berkeleybiolabs.com/ www.bamh1.com www.linkedin.com/in/bethencourt www.logos-press.com/books/biotechnology_business_development.php

13 years

Sudo Kids 2nite! Theme: Twist Ties!

by Raymond Lai

Hey all sudoers! Tonight's Sudo Kids' theme is Twist Ties! We will b making twist tie art, jewelry and doing some pre-viz for a twist tie stop motion epic (Atom & Iris have kicked around a Twist Tie treatment of Moby Dick or The Rhyme of the Ancient Mariner). So bring your twist ties or pipe cleaners and let's make it happen! Does anyone have a stop motion program? Something with onion skinning? See you tonight 5pm for the start of food and 6:00pm-ish for festivities. Oh, and tonight is the P.O.S.S.E. party. So bring delish-ous-ness! Nom nom nom, Ray

13 years

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