sudo-discuss

sudo-discuss@sudoroom.org

7536 discussions

Re: [sudo-discuss] New OpenTrons OT-2 in Sudo/CCL!

by Patrik D'haeseleer

Hi Keoni - do you have any pipettes for the OT-2? We installed it right on the boundary between CCL and Sudo for now. Once we start doing real experiments, we should move it into the wetlab though. We also happen to have a Biometra TRobot, which is essentially a thermocycler with an automated lid, so it can be easily incorporated into a liquid handling robot. That should give us an ideal platform for PCR applications on the Opentrons... DNA barcoding of mushroom species is probably our #1 killer app for this robot. When Alan Rockefeller comes back from his current outing throughout Mexico, he will likely bring several hundred mushroom samples with him, all of which will need to be submitted to PCR amplification and Sanger sequencing. Alan usually mashes each sample with a little plastic pestle in an Eppendorf tube - not sure how to replicate that on the robot, but we could brainstorm a little device to macerate mushroom samples... Patrik On Sun, Sep 30, 2018 at 6:42 PM Keoni Gandall <koeng101(a)gmail.com> wrote: > The hardware issue should be easily solved with a little hack that > deactivates OpenTrons pipette-verification (it worked before a certain > software update). > > The hope is that the robot can help bridge the gap between the software > folks at SudoRoom and wetware folks in CCL. > > I'll at hands on workshop as well - come find me in the red polo if you'd > like to talk about the machine! > > On Sun, Sep 30, 2018 at 6:34 PM Patrik D'haeseleer <patrikd(a)gmail.com> > wrote: > >> Hi all, >> >> You may have noticed that Keoni Gandall dropped of a brand new (ish) OpenTrons >> OT-2 liquid handling robot <http://opentrons.com/ot-2>recently! This is >> the much smaller and newer sibling to the giant 5'x5' robot CCL has in the >> basement. It is currently sitting in SudoRoom, and may or may not have a >> hardware or electrical issue to be diagnosed first. >> >> Also, if you'd like to learn how to use this machine, OpenTrons is >> running a *FREE HANDS-ON WORKSHOP* to learn how to use the OT-2 tomorrow >> at the Mission Bay Conference Center; Monday 2-4pm. Registration is free, >> but space may run out, so I would recommend you sign up asap: >> >> >> https://www.eventbrite.com/e/opentrons-hands-on-workshop-tickets-49398612580 >> >> Patrik >> >

7 years, 10 months

NO ON KAVANAUGH

by Jake

it's not over!!!! still need to bug Collins, Murkowski, Flake, Manchin go to their website and click "contact" to send them a message! NO ON KAVANAUGH is plenty, they know why https://www.flake.senate.gov/public/index.cfm/contact-jeff https://www.manchin.senate.gov/contact-joe/email-joe https://www.murkowski.senate.gov/contact/email https://www.collins.senate.gov/contact

7 years, 11 months

plasma screens in the dump/donate area

by Cere Davis

Heya, Whoever “donated” these large plasma screens, can you tell me if they are known to work? They weigh some 80 pounds and I can’t easily move them to a power outlet to test. -Cere -- *Cere Davis* Science-Art | Kinetic Sculpture CereDavis.com @ceremona <http://Twitter.com/Ceremona>

7 years, 11 months

Last Month's Income & Expenses

by Omni Commons

7 years, 11 months

Calling all hardware startups: Sheet Acrylic Manufacturing Workshop

by Ruth Grace Wong

Exciting news: This time Mike Zhao, our contract manufacturing expert, is going to come for the Q&A in person! You can read more about him and the contract manufacturing process here: https://medium.com/supplyframe-hardware/shanghais-contract-manufacturing-a-… *Note that due to Meetup.com fees we are increasing the price to $85, but we will honor the previous price of $75 until Sunday October 7th.* ------- Are you manufacturing something with plastic? How much do you expect to pay for your molds? $10,000? Maybe even $100,000? If you are a startup and don't have the capital or the volumes to justify expensive plastic molds, manufacturing out of sheet acrylic might be the answer for you. Myself (Ruth) and Rik are running a sheet acrylic manufacturing workshop. We make the whole process accessible, from teaching joints/cutting/bending to sharing local prototyping contacts and hosting a 30 minute Q&A with a Chinese contract manufacturer. The workshop is from 9:30AM to 12:30PM on Oct 20th at the Noisebridge Hackerspace, and costs $85: http://meetu.ps/e/FRckd/qrLmq/f Feel free to contact me at ruthgracewong(a)gmail.com if you have any questions Best, Ruth

7 years, 11 months

Re: [sudo-discuss] Bioprinting at CCL tonight! 3D printing with living cells

by Patrik D'haeseleer

PS: if you cannot join us in person, you can also join us remotely over Zoom at https://zoom.us/j/820128495 Or, go to https://zoom.us/join and enter meeting ID: 820 128 495 . Or to call in by phone, dial: +1 (415) 762-9988 or +1 (646) 568-7788 Meeting ID: 820 128 495I Patrik On Thu, Sep 27, 2018, 2:49 PM Patrik D'haeseleer <patrikd(a)gmail.com> wrote: > BioPrinting is 3D printing with living cells. Think of printing a new > heart or kidney for a patient needing a transplant! Or a bit more > DIY-accessible: how about printing a n artificial leaf, as a proof of > principle that we can make a functioning (plant) organ? > > As some of you know, I've been heavily involved in the BioPrinter project > at Biocurious for the past several years. And I've been wanting to get a > parallel group of the ground at CCL as well. > > I plan to give an overview of bioprinting in general, go over what we've > done in the Bioprinter group away Biocurious so far, get hands-on with the > current hardware (which I've absconded from BioC for the week), and discuss > plans to restart a Bioprinting group at CCL. > > Lots of overlap with our existing lab robotics and plant bio groups as > well... > > Hope to see you tonight! > > Patrik >

7 years, 11 months

Fwd: Bioprinting at CCL tonight! 3D printing with living cells

by Patrik D'haeseleer

BioPrinting is 3D printing with living cells. Think of printing a new heart or kidney for a patient needing a transplant! Or a bit more DIY-accessible: how about printing an artificial leaf, as a proof of principle that we can make a functioning (plant) organ? As some of you know, I've been heavily involved in the BioPrinter project at Biocurious for the past several years. And I've been wanting to get a parallel group of the ground at CCL as well. I plan to give an overview of bioprinting in general, go over what we've done in the Bioprinter group away Biocurious so far, get hands-on with the current hardware (which I've absconded from BioC for the week), and discuss plans to restart a Bioprinting group at CCL. Lots of overlap with our existing lab robotics and plant bio groups as well... Hope to see you tonight! Patrik

7 years, 11 months

irc exclusion

by Jake

i guess i can't participate in IRC anymore because i can't figure out how to configure SSL on irssi when i try to join sudoroom it says: 20:16 !wilhelm.freenode.net Only users using SSL could join this channel! so i googled SSL irssi and it says: https://freenode.net/kb/answer/irssi /network add -sasl_username <login> -sasl_password <password> -sasl_mechanism PLAIN freenode /server add -auto -net freenode -ssl -ssl_verify chat.freenode.net 6697 /save i'm guessing <login> and <password> are something that i have to type there but I don't know what they are or how to find them. So i can't be in IRC until i figure that out. as if IRC wasn't already exclusionary enough? -jake

7 years, 11 months

free scrap wood on craigslist

by Jake

assorted scrap wood, free know anyone who wants any? https://sfbay.craigslist.org/eby/zip/6706604722.html -jake

7 years, 11 months

Hardware Hacking Tuesday tonight

by Jake

I might miss tonight's edition of Hardware Hacking Tuesdays. Please show up and introduce new people if they show up, and try to have fun without me. -jake

7 years, 11 months

Jump to page:

2026

2025

2024

2023

2022

2021

2020

2019

2018

2017

2016

2015

2014

2013

sudo-discuss